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http://indianaquariumhobbyist.com/community/ :: View topic - Isolating Rhizobium spp(nitrogen fixers) from soil
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Isolating Rhizobium spp(nitrogen fixers) from soil
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aidscontrol
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PostPosted: Fri Mar 10, 2006 9:33 pm Post subject: Isolating Rhizobium spp(nitrogen fixers) from soil Reply with quote

 for  my  model  practicals  i  had  to  isolate  nitogen  fixing  bacteria  from  soil.
 I  had  to  isolate  Rhizobium  and  grow  a  pure  culture  of  it.
 I  will  explain  to  you  how  simple  it  is  to  have  these  helpfull  bacteria  sleeping  in  your  bed.
 
 
 I  diluted  soil  in  distilled  water.Soil  sticking  to  the  roots  of  leguminous  plants(bean  variety)and  groundnut  are  rich  in  Rhizobium  spp.
 
 I  used  this  to  sample  to  inoculate  in  YEMA(Yeast  extarct  mannitol  Agar).
 
 after  24hrs  i  got  a  good  culture.
 
 This  implies  that  the  soil  is  rich  in  nitrogen  fixers.

 
 
 N2  --->nitrates---->nitrites--------->NH3(ammonia).
 
 this  is  done  by  nitrogen  fixing  bacteria.
 
 
 NH3-->nitrites--->nitrate---->N2(atmosphere).
 
 done  by  denitrifying  bacteria.
 
 all  these  bacterai  are  very  cheap  and  not  far  away  from  your  artificial  aquariums.
 
 
 GO  DIG  THEM  UP  AND  DONT  BUY  THEM  IN  SHOPS
 
 
 
 i  will  soon  find  out  how  to  grow  then  in  your  house  and  explain  it  here.
 Till  then  mix  soil  with  water  shake  it  filter  it  and  pour  it  inside  your  tank.
 
 Aidscontrol.
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joyban
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PostPosted: Sat Mar 11, 2006 11:08 am Post subject:  Reply with quote

 It  is  good  to  have  a  technical  guys  amongst  ourselves  to  help  us  find  things  easy...  Smile
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sandeepraghuvanshi
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PostPosted: Sat Mar 11, 2006 11:37 am Post subject:  Reply with quote

 Hi,
 Nice  to  have  you.
 Can  you  help  me  out  in  testing  for  nitrates?
 I  have  a  nitrite  test  kit.  Is  is  said  that  to  test  "nitrates",  you  have  to  first  convert  them  to  nitrites  and  then  do  a  nitrite  test.
 Te  reduce  nitrates  to  nitrites  I  am  using  "Zinc  dust".  But  it  does  not  seem  to  be  working.
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gokulin
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PostPosted: Sat Mar 11, 2006 12:00 pm Post subject: Re: Isolating Rhizobium spp(nitrogen fixers) from soil Reply with quote

                                                   
aidscontrol  wrote:                

 i  will  soon  find  out  how  to  grow  then  in  your  house  and  explain  it  here.
 Till  then  mix  soil  with  water  shake  it  filter  it  and  pour  it  inside  your  tank.
                 

 
 Nice  to  have  you  here.....
 
 This  would  be  of  great  help  to  speed  up  the  cycling....
 
 One  bizarre  question:  Could  this  process  be  put  this  way?  Adding  this  beneficial  bacteria  with  WCs,  can  we  reduce  the  filteration  in  our  tanks  since  we  have  bacteria  rich  water  to  take  care  of  the  ammonia??
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ravindranak
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PostPosted: Sat Mar 11, 2006 5:46 pm Post subject:  Reply with quote

 by  adding  soil  from  legumes,  wont  you  be  polluting  the  tank  water  with  other  compounds  as  well  ?
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sandeepraghuvanshi
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PostPosted: Sat Mar 11, 2006 6:13 pm Post subject: Re: Isolating Rhizobium spp(nitrogen fixers) from soil Reply with quote

 [quote="aidscontrol"]
 Till  then  mix  soil  with  water  shake  it  filter  it  and  pour  it  inside  your  tank.
 
 Aidscontrol.[/quote]

 
 Hey
 I  missed  this  one.  I  hope  you  are  not  serious??
 I  don't  think  pouring  mud  in  tanks  will  solve  anything.
 Nitrifying  bacteria  are  present  everywhere  and  we  try  to  culture  them  in  enough  quantites  in  our  tanks  to  nuetralize  ammonia  etc.
 The  only  shortcut  is  to  drop  percultured  bacteria  in  tank,  which  are  available  at  some  LFS.  However  realibility  of  such  cultures  is  always  doubtful.
 Further  culture  of  bacteria  is  a  serious  process  and  is  to  be  done  by  trained  persons  only,  kindly  do  not  attempt  it  at  home.  Most  of  the  bacteria  are  present  in  our  surroundings  most  of  the  time  but  concerntrated  culture  of  bacteria  are  different  matter
 You  never  know  what  you  might  culture  if  you  are  not  careful
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gopiqpp
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PostPosted: Sun Mar 12, 2006 2:14 am Post subject:  Reply with quote

 Yes  Sandeep  is  absolutely  right.  Pouring  a  filtered  emulsion  of  water  and  garden  soil  into  your  tank  might  introduce  some  Nitrobacter  but  will  also  introduce  Clostridia  species  which  can  be  very  dangerous.  Especially  so  if  you  have  a  cut  in  your  skin  and  you  are  upto  your  elbows  in  your  tank!!  Not  to  mention  Klebsiella  and  Salmonella.  Can  kill  your  fish!  Thumb Down  
 Another  point:  Soil  Bacteria  are  very  resistent  to  antibiotics.
 
 http://www.pubmedcentral.nih.gov/pagerender.fcgi?artid=1319510&pageindex=3#page
 
 So,  If  one  really  wants  to  go  via  this  route  then  most  Agro  Depts  in  India  sell  Pure  Rhizopodium  innocule  to  farmers  at  very  cheap  prices  (subsidized).  Much  safer  and  a  much  higher  percentage  of  the  desired  organism.  It  seems  also  that  there  are  different  strains  of  Rhizopodium  which  are  plant  specific-  meaning  that  the  bacteria  fixing  nitrogen  for  legumes  like  peas  and  clover  wont  be  very  good  for  rice  plants  which  need  another  type..  So  it  does  not  follow  that  the  bacteria  in  your  garden  are  going  to  work  well  in  your  tank.
   Cheer Up
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aidscontrol
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PostPosted: Sun Mar 12, 2006 10:35 am Post subject:  Reply with quote

 yipeee  i  have  lots  to  reply  to.
 hehe
 
 ok  yes  it  is  not  good  to  culture  bacteria    at  home.about  the  clostridum  spp.
 it  is  anerobic(requires  abundant  co2  for  vegetative  from  to  grow  and  reproduce).
 its  the  same  for  c.tetani,c.botulinumand  c.perfringens.
 
 tetanus  can  grow  well  in  deep  wounds  and  crushed  tissue  like  in  accident  victims  and  stab  wounds.
 
 however  like  u  guys  think  botulinum  is  deadly.It  causes  food  poison.
 But  unless  they  get  anerobic  conditions  they  wont  form  vegetative  forms  instead  they  remain  in  their  spore  form(can  resist  120  degree  C).Spores  dont  form  toxins.But  swallowing  spores  is  deadly.
 
 Klebsiella(pneumonia)  and  salmonella(causes  typhoid  fever)
 
 i  had  typhoid  fever  6  mnts  back  so  i  dont  have  to  worry  for  another  6  months.  Very Happy  
 You  get  immunity  for  1  your  after  each  attk.I  dont  know  about  the  fishes  maybe  they  will  die  off  but  try  anyway  Chuckle  Salmonella  typhi  passes  along  with  the  faeces  into  soil.If  u  can  find  soil  that  you  think  is  not  rich  in  you  know  what  feel  free  to  handle  it.Ofcourse  wash  your  hands  with  detol  or  spirit.In  my  lab  we  found  cultures  growing  in  soap.  ROFL  
 Use  spirit  to  wipe  your  hands  its  damm  effective.
 
 for  others  it  is  dangerous  but  if  you    are  living  in  the  city  dont  bother  anitbiotics  are  closeby.  Rock On  
 
 About  contamination  by  other  compunds  from  the  soil........well  the  fish  wont  die  from  trace  amounts.And  almost  all  water  bodies  and  land  close  to
 the  city  is  polluted  so  whats  the  big  deal.
 
 Yes  if  someone  is  selling  pure  culture  of  bacteria  buy  it  directly  but  you  need  diff  species  of  bacteria  ,1  for  converting  no3    to  no2    etc  .  so  whats  the  point  buying  1    species  of  bacteria  alone.And    the  more  you  avoid  commercial  products  the  more  invovative  you  will  get.The  idea  is  to  fill  in  the  gaps  in  the  nitrogen  cycle    in  your  aquarium.
 
 hmm  i  have  never  used  a  nitrite  kit  n  stuff.But  if  u  find  nitrates  in  your  aquarium  you  will  find  nitrites  too.  Thumb Up  
 iam  looking  for  a  way  to  test  nitrites  quantitatively,i  will  let  u  know  if  i  find  something.
 
 http://www.indianaquariumhobbyist.com/community/modules.php?name=Forums&file=viewtopic&t=2819
 
 
 thats  my  tank  and  the  plants  are  doing  fine  now  and  i  dont  run  an  aerator  for  more  than  2  hours.
 The  pH  is  7  now.
 
 Aidscontrol
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aquascapes
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PostPosted: Sun Mar 12, 2006 8:05 pm Post subject:  Reply with quote

                                                   
Quote:                
for  others  it  is  dangerous  but  if  you  are  living  in  the  city  dont  bother  anitbiotics  are  closeby.                  

   Surprised  
 you  will  never  stop  spreading  the  virus  cause  you  sell  antivirus  Chuckle  
 get  serious  dude  -  I'm  sure  people  don't  mind  spending  a  couple  of  hundred  rupees  if  need  be  on  a  water  additive  (though  I  never  needed  anything  like  it)  than  get    Sick
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aidscontrol
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PostPosted: Sun Mar 12, 2006 8:50 pm Post subject:  Reply with quote

 of  course  i  dont  mind.
 
 Just  thought  people  needed  to  know  what  alternatives  await  them.
 
 
                                                 
Quote:                
you  will  never  stop  spreading  the  virus  cause  you  sell  antivirus                  

 
 i  can  stop  the  spread  of  virus  only  if  i  start  selling  antivirus.  Smile  
 
 
 I  think  most  of  you  guys  must  have  explored  ponds  and  ditches  in  your  locality  or  the  beach.All  these  places  are  rich  in  colliform  bacteria(bacteria  present  in  your  intestine  and  spread  through  faeces).I  have  had  experiences  when  after  exploring  a  pond  i  step  out  and  notice  human  faeces.But  it  has  to  be  done  right,you  cant  enter  a  pond  dressed  like  a  Bird  flew  doctor  Chuckle  
 
 
 Aidscontrol
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gopiqpp
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PostPosted: Sun Mar 12, 2006 8:59 pm Post subject:  Reply with quote

                                                   
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i  had  typhoid  fever  6  mnts  back  so  i  dont  have  to  worry  for  another  6  months.                    

 
 I  wasnt  talking  about  S.typhimurium  specifically,  but  it  can  be  present  if  there  has  been  fecal  contamination.  By  the  way  congratulations  on  your  recovery  from  Typhoid.  You  also  find  many  others  like  Salmonella  enteritidis,  the  cause  of  acute  salmonellosis,  etc.  I  think  you  will  find  this  page  quite  illuminating  :
 http://www.triciaswaterdragon.com/salmonella.htm
 
 
                                                 
Quote:                
You  get  immunity  for  1  your  after  each  attk.I  dont  know  about  the  fishes  maybe  they  will  die  off  but  try  anyway                  

 I  wonder  if  Discus,  Arrowana  or  piranha  owners,  or  any  aquarist  who  loves  his  pet  would  appreciate  that  particular  bit  of  advice.
 So  my  friend,  You  had  an  idea,  and  it  wasnt  a  bad  one,  but  now  that  it  has  been  thoroughly  dissected  maybe  you  can  see  that  the  risks  involved  far  outweigh  any  financial  benefits
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aquascapes
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PostPosted: Sun Mar 12, 2006 9:59 pm Post subject:  Reply with quote

                                                   
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So  my  friend,  You  had  an  idea,  and  it  wasnt  a  bad  one,  but  now  that  it  has  been  thoroughly  dissected  maybe  you  can  see  that  the  risks  involved  far  outweigh  any  financial  benefits                

 Good  conclusion  !
 A  bright  idea  -  a  healthy  discussion  -  a  safe  conclusion    Thumb Up
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retro_gk
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PostPosted: Mon Mar 13, 2006 9:33 am Post subject:  Reply with quote

 1.  Set  tank  up.
 2.  Introduce  source  of  ammonia  or  an  aged  filter
 3.  ???
 4.  Profit!!
 
 Far  simpler  than  trying  to  innoculate  a  tank  with  mud.
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oortcloudfish
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PostPosted: Mon Mar 13, 2006 6:49 pm Post subject: neo-pisciculture Reply with quote

 hail  aidscontrol  !
 
 i  wonder  if  anyone's  tried  feeding  fish  with  gecko-droppings.  they  are  rich  in  Moraxella  piscicarbiense  that  are  supposed  to  be  beneficial  for  your  fishes  immune  system.  but  obviously,  it  has  to  be  given  only  as  supplements  and  in  small  amounts  (say  one  pellet  of  the  dropping  each  week).  but  i  dont  think  they  work  on  arrowanas.  too  sensitive  to  changes  in  diet.
 
 adding  mud  to  tanks  is  a  bad  bad  idea.  bad  people.  i  agree.  but  me  has  no  problems  with  the  isolated-rhizobium-put-into-fishtank  hypothesis  that  aidscontrol  has  put  forth  and  hence  enlightened  us  to  a  dawning  era  of  neo-pisciculture.
 
 FISH  ARE  FRIENDS  NOT  FOOD..unless,  of  course,  they  are  on  your  dinner-plate.  Surprised
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aquascapes
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Posts: 2753
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PostPosted: Mon Mar 13, 2006 7:06 pm Post subject:  Reply with quote

                                                   
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but  me  has  no  problems  with  the  isolated-rhizobium-put-into-fishtank  hypothesis  that  aidscontrol  has  put  forth  and  hence  enlightened  us  to  a  dawning  era  of  neo-pisciculture.                  

 if  me  has  no  problem  -  go  ahead  and  try  it!
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