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http://indianaquariumhobbyist.com/community/ :: View topic - Simpler Test Kits ??
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Simpler Test Kits ??
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sandeepraghuvanshi
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PostPosted: Thu Nov 10, 2005 5:39 pm Post subject:  Reply with quote

 Hi,  
 Ammonia  in  water  can  be  tested  by  buying  a  solution  called  "Nesselar’s  reagent".  It  is  a  cheap  reagent  and  is  available  at  all  shops  selling  laboratory  chemicals.
 The  procedure  is  simple.  Take  about  5ml  or  one  teaspoon  of  aquarium  water  in  a  glass  test-tube;  put  3,  4  drops  of  reagent.  Observe  for  5  minutes  if  water  remains  clear  it  means  no  ammonia.
 If  water  turns  any  shade  of  yellow,  ammonia  is  there.  
 I  have  link  to  a  website  which  has  a  color  chart,  in  case  any  body  is  interested.
 A  small  bottle  costs  Rs.  80/-  only,  has  no  expiry  date  and  can  be  used  for  a  long  time.
 I  have  been  using  this  for  last  four  months  and  the  results  are  satisfactory.
 I  am  looking  for  similar  tests  for  nitrite  &  nitrate.
 Hope  this  helps
 Sandeep  R
 Lucknow,
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Suby7272
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PostPosted: Thu Nov 10, 2005 5:49 pm Post subject:  Reply with quote

 Wonderful  post  Sandeep  !!
 Please  post  us  the  link  to  the  color  chart  as  well.
 And  the  price  you  mentioned  for  the  chemical  is  just  too  good.
 "Branded"  testkits  sell  for  almost  5  times  that  price  out  here.
 Hope  Nessler's  Reagent  is  easily  available  in  Bangalore  as  well.
 
 Regards
 
 Suby


Last edited by Suby7272 on Thu Nov 10, 2005 5:51 pm; edited 1 time in total
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rohansd
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PostPosted: Thu Nov 10, 2005 5:50 pm Post subject:  Reply with quote

 Good  Info  Dude  !
   Cheer  
 About  Nitrite  tesiting,
 Please  check  
   this    post    
 
 (refer  gogi's  post  in  there)
 He  talks  about  using  Urine  Testing  Strips  for  Testing  Nitrites.
 The  brand  name  that  he  refers  is  not  available  in  India.  We  have  some  other  brand  name  made  by  Bayer  though.
 
 A  tin  of  25  strips  (dont  remember  exactly)  was  avaliable  for  125  bucks
 I  did  not  pursue  it  further.
 You  can  try  if  u  are  interested.
 
 The  problem  that  i  see  with  such  kits  is  that  we  can  not  have  quantative  results  which  is  necessary  many  a  times.[/url]
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sandeepraghuvanshi
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PostPosted: Thu Nov 10, 2005 6:00 pm Post subject:  Reply with quote

 hi,  
 I  am  surprised  that  reply  is  so  fast  in  this  forum,  people  really  are  dedicated.
 Any  way  the  link  is  http://www.goldfishinfo.com/ammonia.htm.
 This  is  very  cool  website  for  goldfishes.
 I  have  a  large  collection  of  website  links  and  would  be  very  happy  to  post  them.
 regards
 Sandeep  R
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Suby7272
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PostPosted: Thu Nov 10, 2005 6:13 pm Post subject:  Reply with quote

 Sandeep's  done  his  job.
 Now  all  you  Chemical  Brothers  of  Bangalore  -  please  post  names  and  addresses  of  vendors  whom  we  can  approach  for  our  chemical  needs.
 
 Regards
 
 Suby
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sandeepraghuvanshi
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PostPosted: Thu Nov 10, 2005 6:38 pm Post subject:  Reply with quote

 Hi,
 This  chemical  is  used  in  every  highschool  chemistry  lab,  so  there  should  be  no  problem  in  obtaining  it.  If  you  use  it  kindly  let  me  know  the  results.
 s  Raghuvanshi
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deepesh
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PostPosted: Fri Nov 11, 2005 11:48 pm Post subject:  Reply with quote

 Hi  All  
 
 Sandeep  is  right.  Those  of  us  who  did  science  in  their  plus  one-twos  as  recently  as  ummmm  10  years  back  may  still  remember  that  Nesslers  used  to  be  the  de  jure  test  for  ammonia.
 
 Should  be  simple  since  the  ammonia  level  should  be  zero  and  the  amount  actually  present  is  not  important,  any  detectable  ammonia  should  be  taken  as  problematic.  I  am  saying  this  because  it  is  difficult  to  quantify  with  Nesslers.
 
 Test  are  really  costly  (350  each  in  chandigarh)  and  the  nitrate  kit  needs  the  nitrite  kit,  you  have  to  buy  both  if  you  want  to  test  nitrates.
 
 Hey  does  Bayer  still  have  those  multistix  in  the  market?
 
 Deepesh
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rohansd
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PostPosted: Mon Nov 14, 2005 10:10 am Post subject:  Reply with quote

                                                   
deepesh  wrote:                
Hi  All  
 
 Hey  does  Bayer  still  have  those  multistix  in  the  market?
 
 Deepesh                

 
 Yes,
 They  do  have  it  but  under  a  different  brand  name.
 I  dont  recall  the  name  but  I  have  seen  them  at  a  Medical  Shop.
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Suby7272
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PostPosted: Mon Nov 14, 2005 6:13 pm Post subject:  Reply with quote

 Hi  guys
 
 Today  I  went  looking  for  Urine  Testing  Strips  by  Bayar.  
 One  of  the  pharmacist  had  this,  but  from  it's  label  it  appeared  as  though  it  checked  for  Glucose  and  not  Nitrites.  So,  I  didnt  pick  that  up.  Did  I  do  it  right  ?  Or,  is  that  the  same  thing  that  we  are  talking  about  ??
 Is  there  any  particular  name  that  I  should  ask  for  ?
 
 Regards
 
 Suby
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discusboy
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PostPosted: Mon Nov 14, 2005 6:17 pm Post subject:  Reply with quote

                                                   
rohansd  wrote:                

 A  tin  of  25  strips  (dont  remember  exactly)  was  avaliable  for  125  bucks
 I  did  not  pursue  it  further.
 You  can  try  if  u  are  interested.
 
 The  problem  that  i  see  with  such  kits  is  that  we  can  not  have  quantative  results  which  is  necessary  many  a  times.[/url]                

 
 what  if  u  get  test  kits  which  give  u  the  accurate  result  and  at  the  same  price  or  a  rupee  or  2  rs  more  per  test  ..
 
 by  using  the  prod  stated  by  u  one  test  will  cost  5  bucks.  
 
 I  will  post  the  details  of  test  kits  by  tomorrow...that  is  if  u  r  interested...
 
 Let  me  know..
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Suby7272
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PostPosted: Mon Nov 14, 2005 6:33 pm Post subject:  Reply with quote

                                                   
discusboy  wrote:                

 what  if  u  get  test  kits  which  give  u  the  accurate  result  and  at  the  same  price  or  a  rupee  or  2  rs  more  per  test  ..
 
 by  using  the  prod  stated  by  u  one  test  will  cost  5  bucks.  
 
 I  will  post  the  details  of  test  kits  by  tomorrow...that  is  if  u  r  interested...
 
 Let  me  know..                

 
 Neki  aur  pooch  pooch  ??    Chuckle  
 (Translation  :  "Why  ask  when  you  are  doing  something  good")
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rohansd
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PostPosted: Mon Nov 14, 2005 6:51 pm Post subject:  Reply with quote

 Suby
 I  cannot  recall  what  did  i  read  on  the  tin  of  the  strips  that  i  was  looking  to  buy.
 Let  me  check  it  again  and  then  i  will  let  u  know.
 
 Mitul.
 Please  let  us  know  abt  the  test  kits  that  u  are  talking  abt.
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discusboy
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PostPosted: Tue Nov 15, 2005 3:55 pm Post subject:  Reply with quote

                                                   
Suby7272  wrote:                
                                                 
discusboy  wrote:                

 what  if  u  get  test  kits  which  give  u  the  accurate  result  and  at  the  same  price  or  a  rupee  or  2  rs  more  per  test  ..
 
 by  using  the  prod  stated  by  u  one  test  will  cost  5  bucks.  
 
 I  will  post  the  details  of  test  kits  by  tomorrow...that  is  if  u  r  interested...
 
 Let  me  know..                

 
 Neki  aur  pooch  pooch  ??    Chuckle  
 (Translation  :  "Why  ask  when  you  are  doing  something  good")                

 
   ROFL  
 
 Wanted  to  be  sure  of  the  availability  and  if  worth  the  effort..  Smile  
 
 I  m  supposed  to  get  the  prices  and  details  ..will  post  in  a  new  thread  as  would  come  under  buy  /sell/ex...as  soon  as  i  get  it...
 
 Bye...
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deepesh
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PostPosted: Fri Nov 18, 2005 9:14 pm Post subject:  Reply with quote

 I  asked  around  for  the  Bayer  multistix.  They  are  pretty  accurate.  You  have  to  be  when  you  are  dealing  with  human  tests  as  these  are  covered  by  liability  laws.  But  the  cost!!!  I  was  quoted  1000  for  125  strips!!  That  I  think  is  quite  correct  for  ketostix  that  we  use  to  test  urine  ketones  cost  525  for  50  strips.  The  multistix  contain  the  ketone  test  too.
 
 Mituls  words  have  got  my  ears  pricked  up.  Very Happy  
 
 Deepsh
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sandeepraghuvanshi
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PostPosted: Tue Nov 29, 2005 5:23 pm Post subject:  Reply with quote

 Hi  guys
 I  dug  this  one  up  as  I  have  found  a  similar  test  for  nitrates.  only  problem  is  that  these  chemicals  are  either  not  available  by  another  name  or  what
 The  local  chemical  stores  now  down  their  shutters  as  soon  as  I  enter  the  market  ROFL  
 Nitrate  Reduction  Test  
 --------------------------------------------------------------------------------
   
 Purpose  This  test  detects  the  ability  of  an  organism  to  reduce  nitrate  (NO3)  to  nitrite  (NO2)  or  some  other  nitogenous  compound,  such  as  molecular  nitrogen  (N2),  using  the  enzyme  nitrate  reductase.  
         
 Principle  Nitrate  (NO3)  may  be  reduced  to  several  different  compounds,  either  by  anaerobic  respiration  or  by  denitrification.  This  test  is  used  to  detect  whether  or  not  the  reduction  has  taken  place.  The  nitrate  medium  contains  potassium  nitrate  as  the  substrate.  If  the  organism  reduces  the  nitrate  to  nitrite,  the  nitrite  will  react  with  added  reagents  sulfanilic  acid  and  a-naphthylamine  to  produce  a  red  color.  If  no  color  is  produced,  this  can  indicate  either  of  two  reactions:  (1)  the  nitrate  was  not  reduced  (2)  the  nitrate  was  reduced  even  further  to  compounds  other  than  nitrite.
 
 To  distinguish  between  the  negative  reaction,  or  the  complete  reduction,  zinc  dust  is  added.  If  nitrate  remains  in  the  medium,  zinc  will  reduce  it  to  nitrate,  and  a  pink  color  is  observed.  This  is  a  negative  reaction.  No  color  change  after  zinc  is  added  means  that  nitrate  has  been  reduced  to  compounds  other  than  nitrite.  This  is  interpreted  as  positive  and  is  often  call  positive  complete  to  distinguish  it  from  the  first  positive  test  discussed.
   
   STEP  ONE
 The  Medium:  Nitrate  Broth
 
 Step  1:
 
 Red  here  indicates  that  the  organism  reduced  the  nitrate  to  nitrite.
       Nitrite  Test  Reagents  Test  for  the  presence  of  nitrite  (NO2)  by  adding  one  dropperful  of  sulfanilic  acid  and  one  dropperful  of  alpha-naphthylamine  reagent.  In  this  step,  a  color  change  to  red  is  a  positive  test  for  the  reduction  of  nitrate  --  it  indicates  that  nitrate  (NO3)  has  been  reduced  to  nitrite  (NO2).  No  color  change  requires  further  testing.  1st  Step:  A  red  color  indicates  the  organism  is  positive  for  reducing  nitrate  to  nitrite.  
 If  there  is  no  color  change,  then  more  testing  must  be  done.
   
   
 
 
   
 
 Reading  Results:  Nitrate  Reduction  Pseudomonas  aeruginosa:  appears  negative
 no  color  change:  must  test  further
 
 
 Escherichia  coli:  positive
 color  change  to  red
 
 
 Corynebacterium  xerosis:  appears  negative
 no  color  change:  must  test  further
   
 
   
 
 --------------------------------------------------------------------------------
   
 STEP  TWO
 Medium:  Nitrate  Broth  (con'd)
 
 
 
 Step  2:
 
 Red  here  indicates  that  the  zinc  reduced  the  nitrate  to  nitrite.
       Testing  for  Unaltered  Nitrate  Test  for  the  presence  of  the  original,  unaltered  nitrate  by  adding  a  small  amount  of  powdered  zinc  to  the  tubes  which  appear  negative  in  the  test  above.  This  is  a  test  to  determine  whether:
           1.  the  clear  color  (above)  means  the  organism  really  did  not  reduce  the  nitrate,  or  whether
           2.  the  clear  color  (above)  means  the  organism  did  nitrate  was  reduced  beyond  nitrite  to  NH2  or  N2.  
 
 With  this  step,  a  red  color  indicates  the  presence  of  nitrite  (caused  by  the  addition  of  zinc).  Therefore:  the  red  color  this  times  indicates  a  the  organism  was  negative  for  nitrate  reduction.  (The  addition  of  zinc  produces  a  chemical  reaction  --  reduction  of  nitrate  to  nitrite.  The  original  nitrate  which  was  unaltered  by  Corynebacterium  xerosis    is  reduced  to  nitrite  by  the  zinc,  not  the  organism.)  No  color  change  indicates  that  the  organism  is  positive  for  nitrate  reduction:  the  original  nitrate  is  no  longer  there;  it  has  been  reduced  past  nitrite  to  NH2  or  N2.  
 
 
 Reading  Results:  Nitrate  Reduction  Pseudomonas  aeruginosa:  positive
 (no  color  change)
 
 Corynebacterium  xerosis:  negative
 (color  change  to  red)
   
 
 
 2nd  step:  a  red  color  here  means  the  organisms  a  negative  for  reduction  of  nitrate  to  nitrite.  In  this  case,  it  was  the  zinc,  and  not  the  organism  which  reduced  the  nitrate.
   
 
   
 
 
       Nitrate  Test  Reagents
   
   
 Reagent  A:  Sulfanilic  Acid
 Reagent  B:  alpha-naphthylamine

     
 Zinc  Dust  is  added  to  tubes  that  appear  to  be  negative  (remain  clear)  after  the  addition  of
 Nitrate  Reagents  A  
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