Posted: Mon Aug 30, 2010 12:05 pm Post subject: Re: Culturing Daphnia...... need some assistance
i had just started with daphnia just a week ago so no much knowledge and experience yet.
but i've been using this guidlines in culturing Daphnias...
1) Set up your green water cultures
Take some clean plastic storage containers or old used aquariums, and place them in a spot outdoors that gets plenty of sunlight, but not too much direct sun. Fill the containers with some gunky water vacuumed from your fish tanks, and toss in a pinch of natural fertilizer such as blood or bone meal. Some daphnia culturists report having good luck using dissolved Miracle Grow at the rate of 1 teaspoon per gallon of water. An old gallon milk jug is perfect for dissolving the mix.
Set up at least two green water containers before you buy your starter culture of daphnia. Allow the green water cultures to become emerald green in color. It’s a very good idea to have duplicate cultures going just in case one of them crashes. It is also a good idea to add a powerhead to the containers to keep the water circulating.
What good is it to take a few weeks storing up a bunch of green water only to have it eaten by the daphnia in a few days? As a rule of thumb, set up at least three times more green water than you need to house your daphnia in. With a couple of containers equaling twelve gallons of green water, you can safely plan on supporting four gallons of daphnia culture. A single gallon container of water can support hundreds of daphnia.
2) Prepare for the arrival of your daphnia culture
Once you have plenty of emerald green water, it is time to transfer some green water culture to the containers that will house your daphnia. To keep a constant supply of green water going, be sure to replace any green water you transfer out of the green water container with tank water or dechlorinated tap water. Try not to use all of your green water up, since it is much more work to start a new culture than it is to keep an existing one going.
For housing daphnia cultures indoors, plastic shoe or sweater boxes work well as does the standard ten-gallon tank. Outdoor cultures do well with 55-gallon drums, plastic tubs or kiddy wading pools. Just about anything that holds water and isn’t toxic can hold daphnia.
It is also a good idea to set up some smaller cultures with a number of different water conditions and different types of containers. Pint-sized drinking water bottles or 2-liter soda pop bottles work well in a pinch. The idea is to hedge your bet by placing your daphnia culture in green water, spring water, treated tap water and whatever else you can think of, to assure that at least some of the daphnia will survive. There is a remote chance that your new arrivals may not take to your green water or your containers may not be daphnia safe. It is better to be cautious by not putting all your eggs in one basket.
3) Order your daphnia culture
You will find a list of reputable vendors at the Aquamaniacs live food forum. We recommend that you ship with next day delivery whenever possible. It’s also a good idea to plan ahead and make sure you are home to receive your daphnia when the delivery truck arrives.
100 to 400 count cultures are pretty standard and should be enough to get you off to a good start.
4) Acclimate and release the daphnia
Open the shipping box immediately. A few dead daphnia in the shipping bag is normal. A lot of dead daphnia should have you in contact with your vendor.
If you notice a marked difference between the temperature of the shipping bag and that of your water containers, you should float the bag for 10 to 15 minutes to equalize the temperature a bit. If both are relatively the same, you can just start divvying up the daphnia amongst the various containers at your disposal. Add a few daphnia at a time, very slowly. Do you see any instant deaths? Don’t put any more daphnia in a container that has them sinking to the bottom to die. Keep divvying them up until they are all spread out among a number of water containers. A dedicated fish room eyedropper works well for transferring the daphnia.
5) Check on your cultures daily
So your new daphnia culture made it through the night in your green water? Good for you! You can start consolidating your mini-cultures into the green water until you have at least two cultures. Again, multiple cultures will help hedge your bet should one of the cultures crash. You will have learned whether daphnia can live in your treated tap water, which is good to know. Daphnia are so sensitive to toxic water that they are used in industry to test for water pollution, sort of like canaries in a coal mine.
Check daily to make sure they have enough green water. Add more as needed, and remember to replenish any outdoor green water containers accordingly if you have them.
6) Care and feeding
If the daphnia eat your green water too fast you may need to set up another green water culture. Daphnia will also eat powdered fish food flakes, bacteria-laden water, and even infusoria from snail droppings. Hikari “First Bites”, “Liquifry”, “Spiralina Powder” and “Cyclop-Eeze” are great foods to supplement your green water as well. Care must be taken not to over feed or pollute the daphnia culture water.
Daphnia populations are known to pulse (rise and fall). A lot depends on water quality, available oxygen, light duration, and available food sources. Some trial and error and experimentation is in order with regard to light duration, added air bubblers and amount of food to offer. By and large, leaving the light on all the time will help promote algae and bacteria in the culture, and an air bubbler (no airstone!) will keep water circulating. The ambient temperature should be kept in a range that is comfortable for people. Outdoor cultures can be pretty much left to their own devices.
If you fear your culture is crashing (you start to see a lot of dead daphnia), remove 10% of your culture water. Then add fresh conditioned water and some food to the container. Also, harvest some of the daphnia to start a new culture, and/or provide a heavy feeding for your fish.
Wait at least a few weeks before you harvest the daphnia from a new culture.
Using a small fish net with large open mesh, take a scoop or two inside the container, capturing some of the adult daphnia. The smaller daphnia will slip through the holes in the net to live another day and grow larger. Always leave some daphnia behind to keep the culture going. Transfer the captured daphnia to a small container of treated tap water for rinsing.
8) Rinse the daphnia before feeding them to your fish
You do not want to add green water or detritus to your tanks! Pour the captured daphnia into a brine shrimp net or coffee gold filter and rinse them gently with dechlorinated water. Offer the daphnia to your fish with a dedicated fish room eyedropper. Overfeeding with any food is not recommended. Remember, a fish’s stomach is the size of its eye.
Posted: Tue Aug 31, 2010 6:11 pm Post subject: Re: Culturing Daphnia...... need some assistance
ahh!!.... dont just whack your brains so much!!..... just take small bucket 10l approx..... add rainwater or aged water(it better be green for the first time)... dump in your culture, put in some yeast, half-tsp, add half tsp sugar.... n leave it.... n add a drop of one mol solution of calcium phosphate weekly..... done!.... remember daphnia doesn tolerate metal ions.... i said calciumphosphate because its good for the reproductive cycle n phosphate causes em to reproduce....
Posted: Sun Apr 17, 2011 5:53 pm Post subject: Re: Culturing Daphnia...... need some assistance
the bigger the daphnia container, the greater the chance of stability. well composted chicken manure works well. cheaper too. but the crashes and booms always happen, so as u said, a single culture is not a good idea.
@samad_cero. interesting technique. does it work all the time?
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