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http://indianaquariumhobbyist.com/community/ :: View topic - Culturing Daphnia...... need some assistance
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Culturing Daphnia...... need some assistance

 
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Samad_Cero
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PostPosted: Sun Aug 08, 2010 1:18 pm Post subject: Culturing Daphnia...... need some assistance Reply with quote

 Hey  everybody....  if  any  of  u  have  experience  keeping  daphnia.....  please  share  your  experience  over  it
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Royston
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PostPosted: Sun Aug 08, 2010 4:05 pm Post subject: Re: Culturing Daphnia...... need some assistance Reply with quote

 I  did  manage  to  keep  it  going  for  more  than  a  couple  of  months  but  frankly  after  knowing  it  was  available  in  Sweet  Home  regularly..why  take  the  trouble.   Cheer
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Samad_Cero
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PostPosted: Sun Aug 08, 2010 9:13 pm Post subject: Re: Culturing Daphnia...... need some assistance Reply with quote

 where  is  this  SWEET  HOME>>>????
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PostPosted: Sun Aug 08, 2010 10:29 pm Post subject: Re: Culturing Daphnia...... need some assistance Reply with quote

 Samad,  i  guess  Daphnia  is  available  in  some  Mumbai  LFS's.  Someone  mentioned  on  another  thread  as  well.
 It  will  be  much  more  easier  to  buy  your  live  food  than  culture  it.
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Samad_Cero
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PostPosted: Tue Aug 10, 2010 4:17 pm Post subject: Re: Culturing Daphnia...... need some assistance Reply with quote

 @alpha91
 ahh...  thanks...  i  had  to  shell  out  100  buck  for  bbs.......  but  my  fry's  loved  it!   Cheering
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PostPosted: Tue Aug 10, 2010 8:37 pm Post subject: Re: Culturing Daphnia...... need some assistance Reply with quote

 Feeding  cultured  live  food  is  always  safe  than  buying  disease  from  the  lfs  for  your  loved  ones.
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PostPosted: Tue Aug 10, 2010 10:35 pm Post subject: Re: Culturing Daphnia...... need some assistance Reply with quote

 I  know  LFS  live  food  does  stand  a  chance  of  carrying  diseases.  
 BUT  you  cannot  just  call  it  "buying  disease"..  
 I  personally  feed  store  bought  tubifex  to  all  fish  everyday,  without  any  problems.
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PostPosted: Wed Aug 11, 2010 12:09 am Post subject: Re: Culturing Daphnia...... need some assistance Reply with quote

 @shubhankar  Its  just  not  a  personal  opinion.may  be  on  your  side  there  may  be  no  problem  as  of  yet.
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damaelle
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PostPosted: Mon Aug 30, 2010 12:05 pm Post subject: Re: Culturing Daphnia...... need some assistance Reply with quote

 i  had  just  started  with  daphnia  just  a  week  ago  so  no  much  knowledge  and  experience  yet.
 
 but  i've  been  using  this  guidlines  in  culturing  Daphnias...
 
 1)  Set  up  your  green  water  cultures
 Take  some  clean  plastic  storage  containers  or  old  used  aquariums,  and  place  them  in  a  spot  outdoors  that  gets  plenty  of  sunlight,  but  not  too  much  direct  sun.  Fill  the  containers  with  some  gunky  water  vacuumed  from  your  fish  tanks,  and  toss  in  a  pinch  of  natural  fertilizer  such  as  blood  or  bone  meal.  Some  daphnia  culturists  report  having  good  luck  using  dissolved  Miracle  Grow  at  the  rate  of  1  teaspoon  per  gallon  of  water.  An  old  gallon  milk  jug  is  perfect  for  dissolving  the  mix.
 
 Set  up  at  least  two  green  water  containers  before  you  buy  your  starter  culture  of  daphnia.  Allow  the  green  water  cultures  to  become  emerald  green  in  color.  It’s  a  very  good  idea  to  have  duplicate  cultures  going  just  in  case  one  of  them  crashes.  It  is  also  a  good  idea  to  add  a  powerhead  to  the  containers  to  keep  the  water  circulating.
 
 What  good  is  it  to  take  a  few  weeks  storing  up  a  bunch  of  green  water  only  to  have  it  eaten  by  the  daphnia  in  a  few  days?  As  a  rule  of  thumb,  set  up  at  least  three  times  more  green  water  than  you  need  to  house  your  daphnia  in.  With  a  couple  of  containers  equaling  twelve  gallons  of  green  water,  you  can  safely  plan  on  supporting  four  gallons  of  daphnia  culture.  A  single  gallon  container  of  water  can  support  hundreds  of  daphnia.
 
 2)  Prepare  for  the  arrival  of  your  daphnia  culture
 Once  you  have  plenty  of  emerald  green  water,  it  is  time  to  transfer  some  green  water  culture  to  the  containers  that  will  house  your  daphnia.  To  keep  a  constant  supply  of  green  water  going,  be  sure  to  replace  any  green  water  you  transfer  out  of  the  green  water  container  with  tank  water  or  dechlorinated  tap  water.  Try  not  to  use  all  of  your  green  water  up,  since  it  is  much  more  work  to  start  a  new  culture  than  it  is  to  keep  an  existing  one  going.
 
 For  housing  daphnia  cultures  indoors,  plastic  shoe  or  sweater  boxes  work  well  as  does  the  standard  ten-gallon  tank.  Outdoor  cultures  do  well  with  55-gallon  drums,  plastic  tubs  or  kiddy  wading  pools.  Just  about  anything  that  holds  water  and  isn’t  toxic  can  hold  daphnia.
 
 It  is  also  a  good  idea  to  set  up  some  smaller  cultures  with  a  number  of  different  water  conditions  and  different  types  of  containers.  Pint-sized  drinking  water  bottles  or  2-liter  soda  pop  bottles  work  well  in  a  pinch.  The  idea  is  to  hedge  your  bet  by  placing  your  daphnia  culture  in  green  water,  spring  water,  treated  tap  water  and  whatever  else  you  can  think  of,  to  assure  that  at  least  some  of  the  daphnia  will  survive.  There  is  a  remote  chance  that  your  new  arrivals  may  not  take  to  your  green  water  or  your  containers  may  not  be  daphnia  safe.  It  is  better  to  be  cautious  by  not  putting  all  your  eggs  in  one  basket.
 
 3)  Order  your  daphnia  culture
 You  will  find  a  list  of  reputable  vendors  at  the  Aquamaniacs  live  food  forum.  We  recommend  that  you  ship  with  next  day  delivery  whenever  possible.  It’s  also  a  good  idea  to  plan  ahead  and  make  sure  you  are  home  to  receive  your  daphnia  when  the  delivery  truck  arrives.
 
 100  to  400  count  cultures  are  pretty  standard  and  should  be  enough  to  get  you  off  to  a  good  start.
 
 4)  Acclimate  and  release  the  daphnia
 Open  the  shipping  box  immediately.  A  few  dead  daphnia  in  the  shipping  bag  is  normal.  A  lot  of  dead  daphnia  should  have  you  in  contact  with  your  vendor.
 
 If  you  notice  a  marked  difference  between  the  temperature  of  the  shipping  bag  and  that  of  your  water  containers,  you  should  float  the  bag  for  10  to  15  minutes  to  equalize  the  temperature  a  bit.  If  both  are  relatively  the  same,  you  can  just  start  divvying  up  the  daphnia  amongst  the  various  containers  at  your  disposal.  Add  a  few  daphnia  at  a  time,  very  slowly.  Do  you  see  any  instant  deaths?  Don’t  put  any  more  daphnia  in  a  container  that  has  them  sinking  to  the  bottom  to  die.  Keep  divvying  them  up  until  they  are  all  spread  out  among  a  number  of  water  containers.  A  dedicated  fish  room  eyedropper  works  well  for  transferring  the  daphnia.
 
 5)  Check  on  your  cultures  daily
 So  your  new  daphnia  culture  made  it  through  the  night  in  your  green  water?  Good  for  you!  You  can  start  consolidating  your  mini-cultures  into  the  green  water  until  you  have  at  least  two  cultures.  Again,  multiple  cultures  will  help  hedge  your  bet  should  one  of  the  cultures  crash.  You  will  have  learned  whether  daphnia  can  live  in  your  treated  tap  water,  which  is  good  to  know.  Daphnia  are  so  sensitive  to  toxic  water  that  they  are  used  in  industry  to  test  for  water  pollution,  sort  of  like  canaries  in  a  coal  mine.
 
 Check  daily  to  make  sure  they  have  enough  green  water.  Add  more  as  needed,  and  remember  to  replenish  any  outdoor  green  water  containers  accordingly  if  you  have  them.
 
 6)  Care  and  feeding
 If  the  daphnia  eat  your  green  water  too  fast  you  may  need  to  set  up  another  green  water  culture.  Daphnia  will  also  eat  powdered  fish  food  flakes,  bacteria-laden  water,  and  even  infusoria  from  snail  droppings.  Hikari  “First  Bites”,  “Liquifry”,  “Spiralina  Powder”  and  “Cyclop-Eeze”  are  great  foods  to  supplement  your  green  water  as  well.  Care  must  be  taken  not  to  over  feed  or  pollute  the  daphnia  culture  water.
 
 Daphnia  populations  are  known  to  pulse  (rise  and  fall).  A  lot  depends  on  water  quality,  available  oxygen,  light  duration,  and  available  food  sources.  Some  trial  and  error  and  experimentation  is  in  order  with  regard  to  light  duration,  added  air  bubblers  and  amount  of  food  to  offer.  By  and  large,  leaving  the  light  on  all  the  time  will  help  promote  algae  and  bacteria  in  the  culture,  and  an  air  bubbler  (no  airstone!)  will  keep  water  circulating.  The  ambient  temperature  should  be  kept  in  a  range  that  is  comfortable  for  people.  Outdoor  cultures  can  be  pretty  much  left  to  their  own  devices.
 
 If  you  fear  your  culture  is  crashing  (you  start  to  see  a  lot  of  dead  daphnia),  remove  10%  of  your  culture  water.  Then  add  fresh  conditioned  water  and  some  food  to  the  container.  Also,  harvest  some  of  the  daphnia  to  start  a  new  culture,  and/or  provide  a  heavy  feeding  for  your  fish.
 
 Wait  at  least  a  few  weeks  before  you  harvest  the  daphnia  from  a  new  culture.
 
 7)  Harvesting
 Using  a  small  fish  net  with  large  open  mesh,  take  a  scoop  or  two  inside  the  container,  capturing  some  of  the  adult  daphnia.  The  smaller  daphnia  will  slip  through  the  holes  in  the  net  to  live  another  day  and  grow  larger.  Always  leave  some  daphnia  behind  to  keep  the  culture  going.  Transfer  the  captured  daphnia  to  a  small  container  of  treated  tap  water  for  rinsing.
 
 8)  Rinse  the  daphnia  before  feeding  them  to  your  fish
 You  do  not  want  to  add  green  water  or  detritus  to  your  tanks!  Pour  the  captured  daphnia  into  a  brine  shrimp  net  or  coffee  gold  filter  and  rinse  them  gently  with  dechlorinated  water.  Offer  the  daphnia  to  your  fish  with  a  dedicated  fish  room  eyedropper.  Overfeeding  with  any  food  is  not  recommended.  Remember,  a  fish’s  stomach  is  the  size  of  its  eye.
 
 
 Got  it  from  this  site  http://aquamaniacs.net/forum/cms_view_article.php?aid=33.  try  it  if  you  interested..  
 hope  it  might  help..
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PostPosted: Tue Aug 31, 2010 6:11 pm Post subject: Re: Culturing Daphnia...... need some assistance Reply with quote

 @daemelle
 
 ahh!!....  dont  just  whack  your  brains  so  much!!.....  just  take  small  bucket  10l  approx.....  add  rainwater  or  aged  water(it  better  be  green  for  the  first  time)...  dump  in  your  culture,  put  in  some  yeast,  half-tsp,  add  half  tsp  sugar....  n  leave  it....  n  add  a  drop  of  one  mol  solution  of  calcium  phosphate  weekly.....  done!....  remember  daphnia  doesn  tolerate  metal  ions....  i  said  calciumphosphate  because  its  good  for  the  reproductive  cycle  n  phosphate  causes  em  to  reproduce....
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PostPosted: Sun Apr 17, 2011 5:53 pm Post subject: Re: Culturing Daphnia...... need some assistance Reply with quote

 @daemelle,  
 
 the  bigger  the  daphnia  container,  the  greater  the  chance  of  stability.  well  composted  chicken  manure  works  well.  cheaper  too.  but  the  crashes  and  booms  always  happen,  so  as  u  said,  a  single  culture  is  not  a  good  idea.  
 
 @samad_cero.  interesting  technique.  does  it  work  all  the  time?
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jerin111111
Regular Poster on IAH
Regular Poster on IAH



Joined: Mar 31, 2011
Posts: 461
Location: hebbal, Bangalore

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PostPosted: Sun Apr 17, 2011 7:05 pm Post subject: Re: Culturing Daphnia...... need some assistance Reply with quote

 @romi:  i  agree  its  kind  of  DIY
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