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http://indianaquariumhobbyist.com/community/ :: View topic - Aquatic Plants Tissue Culture
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Aquatic Plants Tissue Culture
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Severumkid
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PostPosted: Wed Aug 08, 2012 7:45 pm Post subject: Re: Aquatic Plants Tissue Culture Reply with quote

 This  is  extremely  informative.  Fantastic!
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mandragoran
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PostPosted: Wed Aug 08, 2012 8:02 pm Post subject: Re: Aquatic Plants Tissue Culture Reply with quote

 I  noticed  fungus  in  one  this  morning-  lucens,  I  think.  Will  update  when  (if)  I  ever  get  home.
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Madan
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PostPosted: Wed Aug 08, 2012 8:13 pm Post subject: Re: Aquatic Plants Tissue Culture Reply with quote

 Ashwin,  let  it  be  for  a  while,  we  need  a  pic  of  the  fungus  to  recognise  in  the  future.
 Take  a  pic  and  post.
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Ravabi
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PostPosted: Wed Aug 08, 2012 11:00 pm Post subject: Re: Aquatic Plants Tissue Culture Reply with quote

 Now  the  fun  starts     Huh
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PRAVU
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PostPosted: Wed Aug 08, 2012 11:39 pm Post subject: Re: Aquatic Plants Tissue Culture Reply with quote

 A  fungus  will  look  like  round  shaped,  wool  or  cotton  like.  But  the  colour  will  be  mostly  white,  but  there  might  be  other  colors  too.  If  you  start  with  full  plant  like  glosso,  then  it  will  be  easy  to  grow.  But  when  you  start  with  plant  leaf,  it  is  time  consuming  and  a  bit  tough.  The  main  idea  of  PTS  is  to  make  many  plants  from  single  part  of  it.  So,  best  of  luck.  And  semi  liquid  media  is  a  method  in  which  the  plant  will  grow  a  bit  fast  in  my  opinion.
 Thanks.
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rasikanayak
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PostPosted: Thu Aug 09, 2012 6:39 pm Post subject: Re: Aquatic Plants Tissue Culture Reply with quote

 Here  are  the  pics  clicked  at  5.45pm,  9/08/2012.
 
 The  morning  lot  of  Tissue  culture  samples.
 
 
 
 
 
 
 It  does  not  look  like  Fungus.  And  I  have  no  Idea  how  Bacterial  contamination  looks  like.  Is  this  the  Callus,  then?
 
 Regards,
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PRAVU
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PostPosted: Thu Aug 09, 2012 7:01 pm Post subject: Re: Aquatic Plants Tissue Culture Reply with quote

 It  is  not  clear  from  the  picture,  A  callus  is  an  amorphous  mass  of  unorganized  cells,  which  usually  I  have  seen  greenish,  brown  or  black  or  creamy  in  color.  But  it  should  be  like  mass  of  cells  and  amorphous.  Is  the  explant  is  dying?  What  is  the  creamy  colored  part?  Is  it  like  a  part  of  the  explant  loosing  its  green  color?  If  yes,  then  the  explant  might  be  dying.  If  the  creamy  colored  part  is  a  separate  thing  than  the  explant,  then  it  is  a  contamination.  I  can't  make  it  from  the  figure  as  it  is  blurred.  Also  look  whether  the  creamy  part  is  growing  on  the  surface  of  the  media  or  not,  if  yes  then  it  is  a  contamination.  Because   callus  will  be  a  solid  thing  without  having  any  kind  of  shape.  It  should  be  like  a  clump.
 Thanks.
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rasikanayak
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PostPosted: Thu Aug 09, 2012 7:21 pm Post subject: Re: Aquatic Plants Tissue Culture Reply with quote

 Prabhuddha,
 
 Thanks.  Will  try  to  take  a  better  picture.
 
 Regards,
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PRAVU
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PostPosted: Thu Aug 09, 2012 7:25 pm Post subject: Re: Aquatic Plants Tissue Culture Reply with quote

 http://www.scielo.cl/scielo.php?pid=S0718-58392009000300020&script=sci_arttext
 
   You  can  find  callus  pictures  in  this  paper.
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Madan
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PostPosted: Thu Aug 09, 2012 7:36 pm Post subject: Re: Aquatic Plants Tissue Culture Reply with quote

 Nayak  is  this  in  only  one  bottle  or  many  ?
 
 If  only  one  then  let's  see  if  the  whole  thing  turns  white,  then  we'll  open  and  photograph.
 1-2   days  should  give  a  indication.
 
 Looks  to  me  like  the  ex  plant  is  dying.
 
 I  don't  see  any  contamination  and  bacteria  /  fungus  growth  at  all.
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PRAVU
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PostPosted: Thu Aug 09, 2012 8:31 pm Post subject: Re: Aquatic Plants Tissue Culture Reply with quote

 Yes,  it  looks  to  me  that  the  explant  is  loosing  green  colour,  that  means  it  is  dying,  other  than  that  there  seems  no  contamination.  you  can  easily  point  out  a  contamination  by  the  fact  that  it  will  grow  day  by  day  and  become  bigger  in  size.
 Thanks.
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Ravabi
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PostPosted: Thu Aug 09, 2012 11:21 pm Post subject: Re: Aquatic Plants Tissue Culture Reply with quote

 http://old.padil.gov.au/pbt/index.php?q=node/29&pbtID=98
 
 Nayak,  This  is  how  a  typical  bacterial  colony  will  look.
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PRAVU
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PostPosted: Fri Aug 10, 2012 12:30 am Post subject: Re: Aquatic Plants Tissue Culture Reply with quote

 Yes,  the  creamy  like  growth  will  be  of  bacteria  in  most  of  the  cases.
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rasikanayak
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PostPosted: Fri Aug 10, 2012 10:31 am Post subject: Re: Aquatic Plants Tissue Culture Reply with quote

                                                   
Madan  wrote  (View  Post):                
Nayak  is  this  in  only  one  bottle  or  many  ?
 
 If  only  one  then  let's  see  if  the  whole  thing  turns  white,  then  we'll  open  and  photograph.
 1-2   days  should  give  a  indication.
 
 Looks  to  me  like  the  ex  plant  is  dying.
 
 I  don't  see  any  contamination  and  bacteria  /  fungus  growth  at  all.                

 Three  of  the  bottles,  in  which  the  ex-plants  have  gone  white  at  the  edges.  Will  keep  till  Monday  as  suggested  by  you  and  then  open  and  take  Picture.
 
 Prabhuddha,
 Thanks  for  the  Callus  picture  link.  No,  it  does  not  look  like  that.  The  Tissue  culture  bottle  is  too  thick,  and  the  sides  have  some  media  struck  on  them.  So  better  picture  is  not  possible  till  I  open  the  bottle.  Will  keep  a  watch  and  do  so  on  Monday.
 
 Ravi,
 Thanks  for  the  Bacterial  Picture  link.  No,  It's  not  Bacteria  either.  Let's  see.
 
 Collected  the  Plants  Hormones  from  Madan  today.  Thank  you,  Sir.
 
 Regards,
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diwan
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PostPosted: Fri Aug 10, 2012 12:44 pm Post subject: Re: Aquatic Plants Tissue Culture Reply with quote

 Out  of  the  five  bottles  collected,  three  of  the  the  explants  have  turned  brown.
 No  sign  of  fungal  infections  in  any  of  the  bottles.
 One  bottle  housing  Cryptocoryne  lucens  is  intact.  
 The  other  explant  has  partially  turned  brown  on  the  edges.
 
 Guys,  I  request   any  of  the  IAHians   with  a  camera  residing  around  BTM  to  take  some  pics  of  the  same  at  my  place.
 (  934  171  28  9  4)
 
 Regards.
 
 Dinesh.
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