Joined: Feb 15, 2010 Posts: 662 Location: Malda, WB
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Posted: Wed Aug 08, 2012 11:39 pm Post subject: Re: Aquatic Plants Tissue Culture
A fungus will look like round shaped, wool or cotton like. But the colour will be mostly white, but there might be other colors too. If you start with full plant like glosso, then it will be easy to grow. But when you start with plant leaf, it is time consuming and a bit tough. The main idea of PTS is to make many plants from single part of it. So, best of luck. And semi liquid media is a method in which the plant will grow a bit fast in my opinion.
Thanks.
Joined: Feb 15, 2010 Posts: 662 Location: Malda, WB
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Posted: Thu Aug 09, 2012 7:01 pm Post subject: Re: Aquatic Plants Tissue Culture
It is not clear from the picture, A callus is an amorphous mass of unorganized cells, which usually I have seen greenish, brown or black or creamy in color. But it should be like mass of cells and amorphous. Is the explant is dying? What is the creamy colored part? Is it like a part of the explant loosing its green color? If yes, then the explant might be dying. If the creamy colored part is a separate thing than the explant, then it is a contamination. I can't make it from the figure as it is blurred. Also look whether the creamy part is growing on the surface of the media or not, if yes then it is a contamination. Because callus will be a solid thing without having any kind of shape. It should be like a clump.
Thanks.
Joined: Feb 15, 2010 Posts: 662 Location: Malda, WB
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Posted: Thu Aug 09, 2012 8:31 pm Post subject: Re: Aquatic Plants Tissue Culture
Yes, it looks to me that the explant is loosing green colour, that means it is dying, other than that there seems no contamination. you can easily point out a contamination by the fact that it will grow day by day and become bigger in size.
Thanks.
If only one then let's see if the whole thing turns white, then we'll open and photograph.
1-2 days should give a indication.
Looks to me like the ex plant is dying.
I don't see any contamination and bacteria / fungus growth at all.
Three of the bottles, in which the ex-plants have gone white at the edges. Will keep till Monday as suggested by you and then open and take Picture.
Prabhuddha,
Thanks for the Callus picture link. No, it does not look like that. The Tissue culture bottle is too thick, and the sides have some media struck on them. So better picture is not possible till I open the bottle. Will keep a watch and do so on Monday.
Ravi,
Thanks for the Bacterial Picture link. No, It's not Bacteria either. Let's see.
Collected the Plants Hormones from Madan today. Thank you, Sir.
Joined: Feb 04, 2008 Posts: 498 Location: Bangalore
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Posted: Fri Aug 10, 2012 12:44 pm Post subject: Re: Aquatic Plants Tissue Culture
Out of the five bottles collected, three of the the explants have turned brown.
No sign of fungal infections in any of the bottles.
One bottle housing Cryptocoryne lucens is intact.
The other explant has partially turned brown on the edges.
Guys, I request any of the IAHians with a camera residing around BTM to take some pics of the same at my place.
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